Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage

We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evalua...

Descripción completa

Detalles Bibliográficos
Autores principales: Rudi, Knut, Hagen, Irina, Johnsrud, Bente Carina, Skjefstad, Guro, Tryland, Ingun
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954551/
https://www.ncbi.nlm.nih.gov/pubmed/20948930
http://dx.doi.org/10.3390/ijerph7093376
_version_ 1782187938845556736
author Rudi, Knut
Hagen, Irina
Johnsrud, Bente Carina
Skjefstad, Guro
Tryland, Ingun
author_facet Rudi, Knut
Hagen, Irina
Johnsrud, Bente Carina
Skjefstad, Guro
Tryland, Ingun
author_sort Rudi, Knut
collection PubMed
description We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.
format Text
id pubmed-2954551
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-29545512010-10-14 Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage Rudi, Knut Hagen, Irina Johnsrud, Bente Carina Skjefstad, Guro Tryland, Ingun Int J Environ Res Public Health Article We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing. Molecular Diversity Preservation International (MDPI) 2010-09 2010-08-31 /pmc/articles/PMC2954551/ /pubmed/20948930 http://dx.doi.org/10.3390/ijerph7093376 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Rudi, Knut
Hagen, Irina
Johnsrud, Bente Carina
Skjefstad, Guro
Tryland, Ingun
Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage
title Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage
title_full Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage
title_fullStr Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage
title_full_unstemmed Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage
title_short Different Length (DL) qPCR for Quantification of Cell Killing by UV-induced DNA Damage
title_sort different length (dl) qpcr for quantification of cell killing by uv-induced dna damage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2954551/
https://www.ncbi.nlm.nih.gov/pubmed/20948930
http://dx.doi.org/10.3390/ijerph7093376
work_keys_str_mv AT rudiknut differentlengthdlqpcrforquantificationofcellkillingbyuvinduceddnadamage
AT hagenirina differentlengthdlqpcrforquantificationofcellkillingbyuvinduceddnadamage
AT johnsrudbentecarina differentlengthdlqpcrforquantificationofcellkillingbyuvinduceddnadamage
AT skjefstadguro differentlengthdlqpcrforquantificationofcellkillingbyuvinduceddnadamage
AT trylandingun differentlengthdlqpcrforquantificationofcellkillingbyuvinduceddnadamage

Ejemplares similares