From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is als...

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Detalles Bibliográficos
Autores principales: Zhang, Jin, Khvorostov, Ivan, Teitell, Michael
Formato: Texto
Lenguaje:English
Publicado: MyJove Corporation 2008
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956217/
https://www.ncbi.nlm.nih.gov/pubmed/19066542
http://dx.doi.org/10.3791/832

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956217/
https://www.ncbi.nlm.nih.gov/pubmed/19066542
http://dx.doi.org/10.3791/832

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