From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel

This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is als...

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Detalles Bibliográficos
Autores principales: Zhang, Jin, Khvorostov, Ivan, Teitell, Michael
Formato: Texto
Lenguaje:English
Publicado: MyJove Corporation 2008
Materias:
Cellular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956217/
https://www.ncbi.nlm.nih.gov/pubmed/19066542
http://dx.doi.org/10.3791/832
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author Zhang, Jin
Khvorostov, Ivan
Teitell, Michael
author_facet Zhang, Jin
Khvorostov, Ivan
Teitell, Michael
author_sort Zhang, Jin
collection PubMed
description This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated.
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spelling pubmed-29562172011-02-16 From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel Zhang, Jin Khvorostov, Ivan Teitell, Michael J Vis Exp Cellular Biology This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is also demonstrated. MyJove Corporation 2008-06-10 /pmc/articles/PMC2956217/ /pubmed/19066542 http://dx.doi.org/10.3791/832 Text en Copyright © 2008, Journal of Visualized Experiments http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Cellular Biology
Zhang, Jin
Khvorostov, Ivan
Teitell, Michael
From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
title From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
title_full From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
title_fullStr From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
title_full_unstemmed From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
title_short From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
title_sort from mefs to matrigel 3: passaging hescs from matrigel onto matrigel
topic Cellular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956217/
https://www.ncbi.nlm.nih.gov/pubmed/19066542
http://dx.doi.org/10.3791/832
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AT khvorostovivan frommefstomatrigel3passaginghescsfrommatrigelontomatrigel
AT teitellmichael frommefstomatrigel3passaginghescsfrommatrigelontomatrigel

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