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From MEFs to Matrigel 3: Passaging hESCs from Matrigel onto Matrigel
This video demonstrates how to maintain the growth of human embryonic stem cells (hESCs) in feeder cell-free conditions and how to continuously passage hESCs in feeder cell-free conditions. Confirmation of hESC pluripotency grown in feeder cell-free conditions by immunofluorescence microscopy is als...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
MyJove Corporation
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956217/ https://www.ncbi.nlm.nih.gov/pubmed/19066542 http://dx.doi.org/10.3791/832 |