Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase

L-asparaginase was extracted from Erwinia carotovora and purified by ammonium sulfate fractionation (60–70%), Sephadex G-100, CM cellulose, and DEAE sephadex chromatography. The apparent Mr of enzyme under nondenaturing and denaturing conditions was 150 kDa and 37 ± 0.5 kDa, respectively. L-asparagi...

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Detalles Bibliográficos
Autores principales: Warangkar, Suchita C., Khobragade, Chandrahas N.
Formato: Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2009
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956972/
https://www.ncbi.nlm.nih.gov/pubmed/21048860
http://dx.doi.org/10.4061/2010/165878

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956972/
https://www.ncbi.nlm.nih.gov/pubmed/21048860
http://dx.doi.org/10.4061/2010/165878

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