Isolation of Specific Clones from Nonarrayed BAC Libraries through Homologous Recombination

We have developed a new approach to screen bacterial artificial chromosome (BAC) libraries by recombination selection. To test this method, we constructed an orangutan BAC library using an E. coli strain (DY380) with temperature inducible homologous recombination (HR) capability. We amplified one li...

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Detalles Bibliográficos
Autores principales: Nefedov, Mikhail, Carbone, Lucia, Field, Matthew, Schein, Jacquie, de Jong, Pieter J.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957146/
https://www.ncbi.nlm.nih.gov/pubmed/20981149
http://dx.doi.org/10.1155/2011/560124

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957146/
https://www.ncbi.nlm.nih.gov/pubmed/20981149
http://dx.doi.org/10.1155/2011/560124

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