Overproduced Brucella abortus PdhS-mCherry forms soluble aggregates in Escherichia coli, partially associating with mobile foci of IbpA-YFP

BACKGROUND: When heterologous recombinant proteins are produced in Escherichia coli, they often precipitate to form insoluble aggregates of unfolded polypeptides called inclusion bodies. These structures are associated with chaperones like IbpA. However, there are reported cases of "non-classic...

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Detalles Bibliográficos
Autores principales: Van der Henst, Charles, Charlier, Caroline, Deghelt, Michaël, Wouters, Johan, Matroule, Jean-Yves, Letesson, Jean-Jacques, De Bolle, Xavier
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957392/
https://www.ncbi.nlm.nih.gov/pubmed/20920169
http://dx.doi.org/10.1186/1471-2180-10-248

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957392/
https://www.ncbi.nlm.nih.gov/pubmed/20920169
http://dx.doi.org/10.1186/1471-2180-10-248

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