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Tn-seq; high-throughput parallel sequencing for fitness and genetic interaction studies in microorganisms

Biological pathways are structured in complex networks of interacting genes. Solving the architecture of such networks may provide valuable information, such as how microorganisms cause disease. Here we present a method (Tn-seq) for accurately determining quantitative genetic interactions on a genom...

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Detalles Bibliográficos
Autores principales: van Opijnen, Tim, Bodi, Kip L., Camilli, Andrew
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957483/
https://www.ncbi.nlm.nih.gov/pubmed/19767758
http://dx.doi.org/10.1038/nmeth.1377