The DNA relaxation activity and covalent complex accumulation of Mycobacterium tuberculosis topoisomerase I can be assayed in Escherichia coli: application for identification of potential FRET-dye labeling sites

BACKGROUND: Mycobacterium tuberculosis topoisomerase I (MtTOP1) and Escherichia coli topoisomerase I have highly homologous transesterification domains, but the two enzymes have distinctly different C-terminal domains. To investigate the structure-function of MtTOP1 and to target its activity for de...

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Detalles Bibliográficos
Autores principales: Narula, Gagandeep, Becker, Jennifer, Cheng, Bokun, Dani, Neil, Abrenica, Maria V, Tse-Dinh, Yuk-Ching
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2958883/
https://www.ncbi.nlm.nih.gov/pubmed/20920291
http://dx.doi.org/10.1186/1471-2091-11-41

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2958883/
https://www.ncbi.nlm.nih.gov/pubmed/20920291
http://dx.doi.org/10.1186/1471-2091-11-41

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