Cargando…

The DNA relaxation activity and covalent complex accumulation of Mycobacterium tuberculosis topoisomerase I can be assayed in Escherichia coli: application for identification of potential FRET-dye labeling sites

BACKGROUND: Mycobacterium tuberculosis topoisomerase I (MtTOP1) and Escherichia coli topoisomerase I have highly homologous transesterification domains, but the two enzymes have distinctly different C-terminal domains. To investigate the structure-function of MtTOP1 and to target its activity for de...

Descripción completa

Detalles Bibliográficos
Autores principales:	Narula, Gagandeep, Becker, Jennifer, Cheng, Bokun, Dani, Neil, Abrenica, Maria V, Tse-Dinh, Yuk-Ching
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2958883/ https://www.ncbi.nlm.nih.gov/pubmed/20920291 http://dx.doi.org/10.1186/1471-2091-11-41

Ejemplares similares

Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol
por: Annamalai, Thirunavukkarasu, et al.
Publicado: (2009)

Residues of E. coli topoisomerase I conserved for interaction with a specific cytosine base to facilitate DNA cleavage
por: Narula, Gagandeep, et al.
Publicado: (2012)

Inhibition of Mg(2+) binding and DNA religation by bacterial topoisomerase I via introduction of an additional positive charge into the active site region
por: Sorokin, Elena P., et al.
Publicado: (2008)

Inhibition of Zn(II) Binding Type IA Topoisomerases by Organomercury Compounds and Hg(II)
por: Cheng, Bokun, et al.
Publicado: (2015)

Experimental and computational investigations of Ser10 and Lys13 in the binding and cleavage of DNA substrates by Escherichia coli DNA topoisomerase I
por: Strahs, Daniel, et al.
Publicado: (2006)

Mutation adjacent to the active site tyrosine can enhance DNA cleavage and cell killing by the TOPRIM Gly to Ser mutant of bacterial topoisomerase I
por: Cheng, Bokun, et al.
Publicado: (2008)

Structural basis for suppression of hypernegative DNA supercoiling by E. coli topoisomerase I
por: Tan, Kemin, et al.
Publicado: (2015)

Biochemical Basis of E. coli Topoisomerase I Relaxation Activity Reduction by Nonenzymatic Lysine Acetylation
por: Zhou, Qingxuan, et al.
Publicado: (2018)

Mechanism of Type IA Topoisomerases
por: Dasgupta, Tumpa, et al.
Publicado: (2020)

Identification of Anziaic Acid, a Lichen Depside from Hypotrachyna sp., as a New Topoisomerase Poison Inhibitor
por: Cheng, Bokun, et al.
Publicado: (2013)

Bacterial topoisomerase I as a target for discovery of antibacterial compounds
por: Tse-Dinh, Yuk-Ching
Publicado: (2009)

Mechanistic insights from structure of Mycobacterium smegmatis topoisomerase I with ssDNA bound to both N- and C-terminal domains
por: Cao, Nan, et al.
Publicado: (2020)

Direct Observation of Topoisomerase IA Gate Dynamics
por: Mills, Maria, et al.
Publicado: (2018)

Type IA Topoisomerases as Targets for Infectious Disease Treatments
por: Seddek, Ahmed, et al.
Publicado: (2021)

The role of the Zn(II) binding domain in the mechanism of E. coli DNA topoisomerase I
por: Ahumada, Adriana, et al.
Publicado: (2002)

Resistance to topoisomerase cleavage complex induced lethality in Escherichia coli via titration of transcription regulators PurR and FNR
por: Liu, I-Fen, et al.
Publicado: (2011)

ATM Deficiency Results in Accumulation of DNA-Topoisomerase I Covalent Intermediates in Neural Cells
por: Alagoz, Meryem, et al.
Publicado: (2013)

Localization of Mycobacterium tuberculosis topoisomerase I C-terminal sequence motif required for inhibition by endogenous toxin MazF4
por: Garcia, Pamela K., et al.
Publicado: (2022)

Discovery of novel bacterial topoisomerase I inhibitors by use of in silico docking and in vitro assays
por: Sandhaus, Shayna, et al.
Publicado: (2018)

Microheterogeneity of Topoisomerase IA/IB and Their DNA-Bound States
por: Jeanne Dit Fouque, Kevin, et al.
Publicado: (2019)

Microbial Type IA Topoisomerase C-Terminal Domain Sequence Motifs, Distribution and Combination
por: Diaz, Brenda, et al.
Publicado: (2022)

Deacetylation of topoisomerase I is an important physiological function of E. coli CobB
por: Zhou, Qingxuan, et al.
Publicado: (2017)

Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site
por: Cao, Nan, et al.
Publicado: (2018)

Bacterial topoisomerase I and topoisomerase III relax supercoiled DNA via distinct pathways
por: Terekhova, Ksenia, et al.
Publicado: (2012)

Immunodetection of human topoisomerase I-DNA covalent complexes
por: Patel, Anand G., et al.
Publicado: (2016)

Kinetic Study of DNA Topoisomerases by Supercoiling-Dependent Fluorescence Quenching
por: Wang, Yunke, et al.
Publicado: (2019)

Evaluation of FRET real-time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
por: Safeukui, Innocent, et al.
Publicado: (2008)

Intravital imaging of fluorescent markers and FRET probes by DNA tattooing
por: Bins, Adriaan D, et al.
Publicado: (2007)

Dynamic and facilitated binding of topoisomerase accelerates topological relaxation
por: Michieletto, Davide, et al.
Publicado: (2022)

Topoisomerase IB: a relaxing enzyme for stressed DNA
por: Soren, Bini Chhetri, et al.
Publicado: (2020)

The interaction between transport-segment DNA and topoisomerase IA—crystal structure of MtbTOP1 in complex with both G- and T-segments
por: Ferdous, Shomita, et al.
Publicado: (2022)

A Bayesian method for inferring quantitative information from FRET data
por: Lichten, Catherine A, et al.
Publicado: (2011)

Intracellular localization and interaction of mRNA binding proteins as detected by FRET
por: David Gerecht, Pamela S, et al.
Publicado: (2010)

Specificity of DNA triple helix formation analyzed by a FRET assay
por: Reither, Sabine, et al.
Publicado: (2002)

A novel FRET pair for detection of parallel DNA triplexes by the LightCycler
por: Schneider, Uffe V, et al.
Publicado: (2010)

Analysis of the eukaryotic topoisomerase II DNA gate: a single-molecule FRET and structural perspective
por: Collins, Tammy R. L., et al.
Publicado: (2009)

Etoposide Quinone Is a Covalent Poison of Human Topoisomerase IIβ
por: Smith, Nicholas A., et al.
Publicado: (2014)

RecA can stimulate the relaxation activity of topoisomerase I: Molecular basis of topoisomerase-mediated genome-wide transcriptional responses in Escherichia coli
por: Reckinger, Amy R., et al.
Publicado: (2007)

Tagging and catching: rapid isolation and efficient labeling of organelles using the covalent Spy-System in planta
por: Lang, Martina, et al.
Publicado: (2020)

No Time to Relax and Unwind: Exploration of Topoisomerases and a Growing Field of Study
por: Deweese, Joseph E., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_l1fl3ii35nnm48m2cj623qv99a