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Gene Expression Profiling of Skeletal Muscle of Nursing Piglets

To gain insight into the regulation mechanism associated with the rapid gain in skeletal muscle during neonatal period, gene expression profiles of skeletal muscle of nursing pigs was investigated using Affymetrix Porcine GeneChip. A total of 1094 transcripts were detected as differential expression...

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Autores principales: Jiang, Caode, Shi, Ping, Li, Shun, Dong, Ranran, Tian, Jiawei, Wei, Jin, Luo, Shuang
Formato: Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962265/
https://www.ncbi.nlm.nih.gov/pubmed/20975821
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author Jiang, Caode
Shi, Ping
Li, Shun
Dong, Ranran
Tian, Jiawei
Wei, Jin
Luo, Shuang
author_facet Jiang, Caode
Shi, Ping
Li, Shun
Dong, Ranran
Tian, Jiawei
Wei, Jin
Luo, Shuang
author_sort Jiang, Caode
collection PubMed
description To gain insight into the regulation mechanism associated with the rapid gain in skeletal muscle during neonatal period, gene expression profiles of skeletal muscle of nursing pigs was investigated using Affymetrix Porcine GeneChip. A total of 1094 transcripts were detected as differential expression over time course tested (p<0.01, q<0.05). With combinative use of partitioning around medoid and hierarchical clustering, three clusters of transcripts with distinct temporal expression were defined. Gene functional categories and pathways, particularly involved in cell signaling, cell cycle, cell adhesion, ECM-receptor interaction, glycolysis, protein synthesis and degradation, and intracellular transport, were identified. Moreover, we showed 49 of the differentially expressed genes within published QTL regions or with marked deletion effects. Our study demonstrates previously uncharacterized changes in transcription accompanying early postnatal growth of skeletal muscle of pigs. It has highlighted potential cascades and important candidates for further investigation on controlling of postnatal muscle growth.
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spelling pubmed-29622652010-10-25 Gene Expression Profiling of Skeletal Muscle of Nursing Piglets Jiang, Caode Shi, Ping Li, Shun Dong, Ranran Tian, Jiawei Wei, Jin Luo, Shuang Int J Biol Sci Research Paper To gain insight into the regulation mechanism associated with the rapid gain in skeletal muscle during neonatal period, gene expression profiles of skeletal muscle of nursing pigs was investigated using Affymetrix Porcine GeneChip. A total of 1094 transcripts were detected as differential expression over time course tested (p<0.01, q<0.05). With combinative use of partitioning around medoid and hierarchical clustering, three clusters of transcripts with distinct temporal expression were defined. Gene functional categories and pathways, particularly involved in cell signaling, cell cycle, cell adhesion, ECM-receptor interaction, glycolysis, protein synthesis and degradation, and intracellular transport, were identified. Moreover, we showed 49 of the differentially expressed genes within published QTL regions or with marked deletion effects. Our study demonstrates previously uncharacterized changes in transcription accompanying early postnatal growth of skeletal muscle of pigs. It has highlighted potential cascades and important candidates for further investigation on controlling of postnatal muscle growth. Ivyspring International Publisher 2010-10-11 /pmc/articles/PMC2962265/ /pubmed/20975821 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Jiang, Caode
Shi, Ping
Li, Shun
Dong, Ranran
Tian, Jiawei
Wei, Jin
Luo, Shuang
Gene Expression Profiling of Skeletal Muscle of Nursing Piglets
title Gene Expression Profiling of Skeletal Muscle of Nursing Piglets
title_full Gene Expression Profiling of Skeletal Muscle of Nursing Piglets
title_fullStr Gene Expression Profiling of Skeletal Muscle of Nursing Piglets
title_full_unstemmed Gene Expression Profiling of Skeletal Muscle of Nursing Piglets
title_short Gene Expression Profiling of Skeletal Muscle of Nursing Piglets
title_sort gene expression profiling of skeletal muscle of nursing piglets
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962265/
https://www.ncbi.nlm.nih.gov/pubmed/20975821
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