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Gene Expression Profiling of Skeletal Muscle of Nursing Piglets
To gain insight into the regulation mechanism associated with the rapid gain in skeletal muscle during neonatal period, gene expression profiles of skeletal muscle of nursing pigs was investigated using Affymetrix Porcine GeneChip. A total of 1094 transcripts were detected as differential expression...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962265/ https://www.ncbi.nlm.nih.gov/pubmed/20975821 |
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author | Jiang, Caode Shi, Ping Li, Shun Dong, Ranran Tian, Jiawei Wei, Jin Luo, Shuang |
author_facet | Jiang, Caode Shi, Ping Li, Shun Dong, Ranran Tian, Jiawei Wei, Jin Luo, Shuang |
author_sort | Jiang, Caode |
collection | PubMed |
description | To gain insight into the regulation mechanism associated with the rapid gain in skeletal muscle during neonatal period, gene expression profiles of skeletal muscle of nursing pigs was investigated using Affymetrix Porcine GeneChip. A total of 1094 transcripts were detected as differential expression over time course tested (p<0.01, q<0.05). With combinative use of partitioning around medoid and hierarchical clustering, three clusters of transcripts with distinct temporal expression were defined. Gene functional categories and pathways, particularly involved in cell signaling, cell cycle, cell adhesion, ECM-receptor interaction, glycolysis, protein synthesis and degradation, and intracellular transport, were identified. Moreover, we showed 49 of the differentially expressed genes within published QTL regions or with marked deletion effects. Our study demonstrates previously uncharacterized changes in transcription accompanying early postnatal growth of skeletal muscle of pigs. It has highlighted potential cascades and important candidates for further investigation on controlling of postnatal muscle growth. |
format | Text |
id | pubmed-2962265 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-29622652010-10-25 Gene Expression Profiling of Skeletal Muscle of Nursing Piglets Jiang, Caode Shi, Ping Li, Shun Dong, Ranran Tian, Jiawei Wei, Jin Luo, Shuang Int J Biol Sci Research Paper To gain insight into the regulation mechanism associated with the rapid gain in skeletal muscle during neonatal period, gene expression profiles of skeletal muscle of nursing pigs was investigated using Affymetrix Porcine GeneChip. A total of 1094 transcripts were detected as differential expression over time course tested (p<0.01, q<0.05). With combinative use of partitioning around medoid and hierarchical clustering, three clusters of transcripts with distinct temporal expression were defined. Gene functional categories and pathways, particularly involved in cell signaling, cell cycle, cell adhesion, ECM-receptor interaction, glycolysis, protein synthesis and degradation, and intracellular transport, were identified. Moreover, we showed 49 of the differentially expressed genes within published QTL regions or with marked deletion effects. Our study demonstrates previously uncharacterized changes in transcription accompanying early postnatal growth of skeletal muscle of pigs. It has highlighted potential cascades and important candidates for further investigation on controlling of postnatal muscle growth. Ivyspring International Publisher 2010-10-11 /pmc/articles/PMC2962265/ /pubmed/20975821 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Jiang, Caode Shi, Ping Li, Shun Dong, Ranran Tian, Jiawei Wei, Jin Luo, Shuang Gene Expression Profiling of Skeletal Muscle of Nursing Piglets |
title | Gene Expression Profiling of Skeletal Muscle of Nursing Piglets |
title_full | Gene Expression Profiling of Skeletal Muscle of Nursing Piglets |
title_fullStr | Gene Expression Profiling of Skeletal Muscle of Nursing Piglets |
title_full_unstemmed | Gene Expression Profiling of Skeletal Muscle of Nursing Piglets |
title_short | Gene Expression Profiling of Skeletal Muscle of Nursing Piglets |
title_sort | gene expression profiling of skeletal muscle of nursing piglets |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962265/ https://www.ncbi.nlm.nih.gov/pubmed/20975821 |
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