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Affinity Purification of an Archaeal DNA Replication Protein Network

Nineteen Thermococcus kodakarensis strains have been constructed, each of which synthesizes a different His(6)-tagged protein known or predicted to be a component of the archaeal DNA replication machinery. Using the His(6)-tagged proteins, stable complexes assembled in vivo have been isolated direct...

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Detalles Bibliográficos
Autores principales: Li, Zhuo, Santangelo, Thomas J., Čuboňová, Ľubomíra, Reeve, John N., Kelman, Zvi
Formato: Texto
Lenguaje:English
Publicado: American Society of Microbiology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2962436/
https://www.ncbi.nlm.nih.gov/pubmed/20978540
http://dx.doi.org/10.1128/mBio.00221-10