Microbiome Profiling by Illumina Sequencing of Combinatorial Sequence-Tagged PCR Products

We developed a low-cost, high-throughput microbiome profiling method that uses combinatorial sequence tags attached to PCR primers that amplify the rRNA V6 region. Amplified PCR products are sequenced using an Illumina paired-end protocol to generate millions of overlapping reads. Combinatorial sequ...

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Detalles Bibliográficos
Autores principales: Gloor, Gregory B., Hummelen, Ruben, Macklaim, Jean M., Dickson, Russell J., Fernandes, Andrew D., MacPhee, Roderick, Reid, Gregor
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964327/
https://www.ncbi.nlm.nih.gov/pubmed/21048977
http://dx.doi.org/10.1371/journal.pone.0015406

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964327/
https://www.ncbi.nlm.nih.gov/pubmed/21048977
http://dx.doi.org/10.1371/journal.pone.0015406

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