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Simplified primer design for PCR-based gene targeting and microarray primer database: two web tools for fission yeast
PCR-based gene targeting is a popular method for manipulating yeast genes in their normal chromosomal locations. The manual design of primers, however, can be cumbersome and error-prone. We have developed a straightforward web-based tool that applies user-specified inputs to automate and simplify th...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley & Sons, Ltd.
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964512/ https://www.ncbi.nlm.nih.gov/pubmed/17072893 http://dx.doi.org/10.1002/yea.1422 |