Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli

BACKGROUND: Biotin is an essential enzyme cofactor that acts as a CO(2 )carrier in carboxylation and decarboxylation reactions. The E. coli genome encodes a biosynthetic pathway that produces biotin from pimeloyl-CoA in four enzymatic steps. The final step, insertion of sulfur into desthiobiotin to...

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Detalles Bibliográficos
Autores principales: Delli-Bovi, Teegan A, Spalding, Maroya D, Prigge, Sean T
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964542/
https://www.ncbi.nlm.nih.gov/pubmed/20937134
http://dx.doi.org/10.1186/1472-6750-10-73

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964542/
https://www.ncbi.nlm.nih.gov/pubmed/20937134
http://dx.doi.org/10.1186/1472-6750-10-73

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