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Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm

BACKGROUND: Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) method produces allele-specific DNA bands of different lengths by adding four designed primers and it achieves the single nucleotide polymorphism (SNP) genotyping by electrophoresis without further steps. It is a time...

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Detalles Bibliográficos
Autores principales: Yang, Cheng-Hong, Cheng, Yu-Huei, Chuang, Li-Yeh, Chang, Hsueh-Wei
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964683/
https://www.ncbi.nlm.nih.gov/pubmed/20942913
http://dx.doi.org/10.1186/1471-2105-11-509