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Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm
BACKGROUND: Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) method produces allele-specific DNA bands of different lengths by adding four designed primers and it achieves the single nucleotide polymorphism (SNP) genotyping by electrophoresis without further steps. It is a time...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964683/ https://www.ncbi.nlm.nih.gov/pubmed/20942913 http://dx.doi.org/10.1186/1471-2105-11-509 |