Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography

A simple, fast, specific, and precise high-performance thin layer chromatography method has been developed for the estimation of monosodium l-glutamate (MSG) in food products. Aluminum plates precoated with silica gel 60 GF(254)were used as stationary phase and a mixture of methanol–chloroform–formi...

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Autores principales: Krishna, Veni N, Karthika, D, Surya, Devi M, Rubini, MF, Vishalini, M, Pradeepa, YJ
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2010
Materias:
Pharm Analysis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964770/
https://www.ncbi.nlm.nih.gov/pubmed/21042489
http://dx.doi.org/10.4103/0975-1483.66795
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author Krishna, Veni N
Karthika, D
Surya, Devi M
Rubini, MF
Vishalini, M
Pradeepa, YJ
author_facet Krishna, Veni N
Karthika, D
Surya, Devi M
Rubini, MF
Vishalini, M
Pradeepa, YJ
author_sort Krishna, Veni N
collection PubMed
description A simple, fast, specific, and precise high-performance thin layer chromatography method has been developed for the estimation of monosodium l-glutamate (MSG) in food products. Aluminum plates precoated with silica gel 60 GF(254)were used as stationary phase and a mixture of methanol–chloroform–formic acid in the ratio 5:5:1 (v/v) as mobile phase. Quantification was carried out by postchromatographic derivatization using 1% ninhydrin solution, and the developed spots were scanned by using a densitometer in absorbance mode at 485 nM. The R(f)value of MSG was 0.64. The results of the analysis have been validated statistically and by the recovery studies. Linearity was observed in the concentration range of 400–1000 nG.
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spelling pubmed-29647702010-11-01 Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography Krishna, Veni N Karthika, D Surya, Devi M Rubini, MF Vishalini, M Pradeepa, YJ J Young Pharm Pharm Analysis A simple, fast, specific, and precise high-performance thin layer chromatography method has been developed for the estimation of monosodium l-glutamate (MSG) in food products. Aluminum plates precoated with silica gel 60 GF(254)were used as stationary phase and a mixture of methanol–chloroform–formic acid in the ratio 5:5:1 (v/v) as mobile phase. Quantification was carried out by postchromatographic derivatization using 1% ninhydrin solution, and the developed spots were scanned by using a densitometer in absorbance mode at 485 nM. The R(f)value of MSG was 0.64. The results of the analysis have been validated statistically and by the recovery studies. Linearity was observed in the concentration range of 400–1000 nG. Medknow Publications 2010 /pmc/articles/PMC2964770/ /pubmed/21042489 http://dx.doi.org/10.4103/0975-1483.66795 Text en © Journal of Young Pharmacists http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Pharm Analysis
Krishna, Veni N
Karthika, D
Surya, Devi M
Rubini, MF
Vishalini, M
Pradeepa, YJ
Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography
title Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography
title_full Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography
title_fullStr Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography
title_full_unstemmed Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography
title_short Analysis of Monosodium l-Glutamate in Food Products by High-Performance Thin Layer Chromatography
title_sort analysis of monosodium l-glutamate in food products by high-performance thin layer chromatography
topic Pharm Analysis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2964770/
https://www.ncbi.nlm.nih.gov/pubmed/21042489
http://dx.doi.org/10.4103/0975-1483.66795
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