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New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA

Two archaeal tRNA methyltransferases belonging to the SPOUT superfamily and displaying unexpected activities are identified. These enzymes are orthologous to the yeast Trm10p methyltransferase, which catalyses the formation of 1-methylguanosine at position 9 of tRNA. In contrast, the Trm10p ortholog...

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Autores principales: Kempenaers, Morgane, Roovers, Martine, Oudjama, Yamina, Tkaczuk, Karolina L., Bujnicki, Janusz M., Droogmans, Louis
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965216/
https://www.ncbi.nlm.nih.gov/pubmed/20525789
http://dx.doi.org/10.1093/nar/gkq451
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author Kempenaers, Morgane
Roovers, Martine
Oudjama, Yamina
Tkaczuk, Karolina L.
Bujnicki, Janusz M.
Droogmans, Louis
author_facet Kempenaers, Morgane
Roovers, Martine
Oudjama, Yamina
Tkaczuk, Karolina L.
Bujnicki, Janusz M.
Droogmans, Louis
author_sort Kempenaers, Morgane
collection PubMed
description Two archaeal tRNA methyltransferases belonging to the SPOUT superfamily and displaying unexpected activities are identified. These enzymes are orthologous to the yeast Trm10p methyltransferase, which catalyses the formation of 1-methylguanosine at position 9 of tRNA. In contrast, the Trm10p orthologue from the crenarchaeon Sulfolobus acidocaldarius forms 1-methyladenosine at the same position. Even more surprisingly, the Trm10p orthologue from the euryarchaeon Thermococcus kodakaraensis methylates the N(1)-atom of either adenosine or guanosine at position 9 in different tRNAs. This is to our knowledge the first example of a tRNA methyltransferase with a broadened nucleoside recognition capability. The evolution of tRNA methyltransferases methylating the N(1) atom of a purine residue is discussed.
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spelling pubmed-29652162010-10-28 New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA Kempenaers, Morgane Roovers, Martine Oudjama, Yamina Tkaczuk, Karolina L. Bujnicki, Janusz M. Droogmans, Louis Nucleic Acids Res Molecular Biology Two archaeal tRNA methyltransferases belonging to the SPOUT superfamily and displaying unexpected activities are identified. These enzymes are orthologous to the yeast Trm10p methyltransferase, which catalyses the formation of 1-methylguanosine at position 9 of tRNA. In contrast, the Trm10p orthologue from the crenarchaeon Sulfolobus acidocaldarius forms 1-methyladenosine at the same position. Even more surprisingly, the Trm10p orthologue from the euryarchaeon Thermococcus kodakaraensis methylates the N(1)-atom of either adenosine or guanosine at position 9 in different tRNAs. This is to our knowledge the first example of a tRNA methyltransferase with a broadened nucleoside recognition capability. The evolution of tRNA methyltransferases methylating the N(1) atom of a purine residue is discussed. Oxford University Press 2010-10 2010-06-04 /pmc/articles/PMC2965216/ /pubmed/20525789 http://dx.doi.org/10.1093/nar/gkq451 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Molecular Biology
Kempenaers, Morgane
Roovers, Martine
Oudjama, Yamina
Tkaczuk, Karolina L.
Bujnicki, Janusz M.
Droogmans, Louis
New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA
title New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA
title_full New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA
title_fullStr New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA
title_full_unstemmed New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA
title_short New archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of tRNA
title_sort new archaeal methyltransferases forming 1-methyladenosine or 1-methyladenosine and 1-methylguanosine at position 9 of trna
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2965216/
https://www.ncbi.nlm.nih.gov/pubmed/20525789
http://dx.doi.org/10.1093/nar/gkq451
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