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Analysis of conditional gene deletion using probe based Real-Time PCR

BACKGROUND: Conditional gene deletion using Cre-lox recombination is frequently used in mouse genetics; however recombination is frequently incomplete, resulting in a mixture of cells containing the functional (2lox) allele and the truncated (1lox) allele. Conventional analysis of 1lox/2lox allele r...

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Detalles Bibliográficos
Autores principales: Weis, Britta, Schmidt, Joachim, Lyko, Frank, Linhart, Heinz G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966447/
https://www.ncbi.nlm.nih.gov/pubmed/20950424
http://dx.doi.org/10.1186/1472-6750-10-75