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Analysis of conditional gene deletion using probe based Real-Time PCR

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BACKGROUND: Conditional gene deletion using Cre-lox recombination is frequently used in mouse genetics; however recombination is frequently incomplete, resulting in a mixture of cells containing the functional (2lox) allele and the truncated (1lox) allele. Conventional analysis of 1lox/2lox allele r...

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Detalles Bibliográficos
Autores principales:	Weis, Britta, Schmidt, Joachim, Lyko, Frank, Linhart, Heinz G
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2966447/ https://www.ncbi.nlm.nih.gov/pubmed/20950424 http://dx.doi.org/10.1186/1472-6750-10-75

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