Quantitative dynamic footprinting microscopy reveals mechanisms of neutrophil rolling

We introduce quantitative dynamic footprinting microscopy to resolve neutrophil rolling on P-selectin. We show that the footprint of a rolling neutrophil is four times larger than previously thought, the P-selectin-PSGL-1 bonds are relaxed at the leading edge of the rolling cell, compressed under the cell center, and stretched at the trailing edge. Each rolling neutrophil also forms 3-4 long tethers that extend up to 16 μm behind the rolling cell.