Gene optimization mechanisms: A multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli

The genetic code is universal, but recombinant protein expression in heterologous systems is often hampered by divergent codon usage. Here, we demonstrate that reprogramming by standardized multi-parameter gene optimization software and de novo gene synthesis is a suitable general strategy to improv...

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Detalles Bibliográficos
Autores principales: Maertens, Barbara, Spriestersbach, Anne, von Groll, Uritza, Roth, Udo, Kubicek, Jan, Gerrits, Michael, Graf, Marcus, Liss, Michael, Daubert, Daniela, Wagner, Ralf, Schäfer, Frank
Formato: Texto
Lenguaje:English
Publicado: Wiley Subscription Services, Inc., A Wiley Company 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2970903/
https://www.ncbi.nlm.nih.gov/pubmed/20506237
http://dx.doi.org/10.1002/pro.408

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2970903/
https://www.ncbi.nlm.nih.gov/pubmed/20506237
http://dx.doi.org/10.1002/pro.408

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