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Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium

The relative roles of the endosomal TLR3/7/8 versus the intracellular RNA helicases RIG-I and MDA5 in viral infection is much debated. We investigated the roles of each pattern recognition receptor in rhinovirus infection using primary bronchial epithelial cells. TLR3 was constitutively expressed; h...

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Autores principales: Slater, Louise, Bartlett, Nathan W., Haas, Jennifer J., Zhu, Jie, Message, Simon D., Walton, Ross P., Sykes, Annemarie, Dahdaleh, Samer, Clarke, Deborah L., Belvisi, Maria G., Kon, Onn M., Fujita, Takashi, Jeffery, Peter K., Johnston, Sebastian L., Edwards, Michael R.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2973831/
https://www.ncbi.nlm.nih.gov/pubmed/21079690
http://dx.doi.org/10.1371/journal.ppat.1001178
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author Slater, Louise
Bartlett, Nathan W.
Haas, Jennifer J.
Zhu, Jie
Message, Simon D.
Walton, Ross P.
Sykes, Annemarie
Dahdaleh, Samer
Clarke, Deborah L.
Belvisi, Maria G.
Kon, Onn M.
Fujita, Takashi
Jeffery, Peter K.
Johnston, Sebastian L.
Edwards, Michael R.
author_facet Slater, Louise
Bartlett, Nathan W.
Haas, Jennifer J.
Zhu, Jie
Message, Simon D.
Walton, Ross P.
Sykes, Annemarie
Dahdaleh, Samer
Clarke, Deborah L.
Belvisi, Maria G.
Kon, Onn M.
Fujita, Takashi
Jeffery, Peter K.
Johnston, Sebastian L.
Edwards, Michael R.
author_sort Slater, Louise
collection PubMed
description The relative roles of the endosomal TLR3/7/8 versus the intracellular RNA helicases RIG-I and MDA5 in viral infection is much debated. We investigated the roles of each pattern recognition receptor in rhinovirus infection using primary bronchial epithelial cells. TLR3 was constitutively expressed; however, RIG-I and MDA5 were inducible by 8–12 h following rhinovirus infection. Bronchial epithelial tissue from normal volunteers challenged with rhinovirus in vivo exhibited low levels of RIG-I and MDA5 that were increased at day 4 post infection. Inhibition of TLR3, RIG-I and MDA5 by siRNA reduced innate cytokine mRNA, and increased rhinovirus replication. Inhibition of TLR3 and TRIF using siRNA reduced rhinovirus induced RNA helicases. Furthermore, IFNAR1 deficient mice exhibited RIG-I and MDA5 induction early during RV1B infection in an interferon independent manner. Hence anti-viral defense within bronchial epithelium requires co-ordinated recognition of rhinovirus infection, initially via TLR3/TRIF and later via inducible RNA helicases.
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spelling pubmed-29738312010-11-15 Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium Slater, Louise Bartlett, Nathan W. Haas, Jennifer J. Zhu, Jie Message, Simon D. Walton, Ross P. Sykes, Annemarie Dahdaleh, Samer Clarke, Deborah L. Belvisi, Maria G. Kon, Onn M. Fujita, Takashi Jeffery, Peter K. Johnston, Sebastian L. Edwards, Michael R. PLoS Pathog Research Article The relative roles of the endosomal TLR3/7/8 versus the intracellular RNA helicases RIG-I and MDA5 in viral infection is much debated. We investigated the roles of each pattern recognition receptor in rhinovirus infection using primary bronchial epithelial cells. TLR3 was constitutively expressed; however, RIG-I and MDA5 were inducible by 8–12 h following rhinovirus infection. Bronchial epithelial tissue from normal volunteers challenged with rhinovirus in vivo exhibited low levels of RIG-I and MDA5 that were increased at day 4 post infection. Inhibition of TLR3, RIG-I and MDA5 by siRNA reduced innate cytokine mRNA, and increased rhinovirus replication. Inhibition of TLR3 and TRIF using siRNA reduced rhinovirus induced RNA helicases. Furthermore, IFNAR1 deficient mice exhibited RIG-I and MDA5 induction early during RV1B infection in an interferon independent manner. Hence anti-viral defense within bronchial epithelium requires co-ordinated recognition of rhinovirus infection, initially via TLR3/TRIF and later via inducible RNA helicases. Public Library of Science 2010-11-04 /pmc/articles/PMC2973831/ /pubmed/21079690 http://dx.doi.org/10.1371/journal.ppat.1001178 Text en Slater et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Slater, Louise
Bartlett, Nathan W.
Haas, Jennifer J.
Zhu, Jie
Message, Simon D.
Walton, Ross P.
Sykes, Annemarie
Dahdaleh, Samer
Clarke, Deborah L.
Belvisi, Maria G.
Kon, Onn M.
Fujita, Takashi
Jeffery, Peter K.
Johnston, Sebastian L.
Edwards, Michael R.
Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium
title Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium
title_full Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium
title_fullStr Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium
title_full_unstemmed Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium
title_short Co-ordinated Role of TLR3, RIG-I and MDA5 in the Innate Response to Rhinovirus in Bronchial Epithelium
title_sort co-ordinated role of tlr3, rig-i and mda5 in the innate response to rhinovirus in bronchial epithelium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2973831/
https://www.ncbi.nlm.nih.gov/pubmed/21079690
http://dx.doi.org/10.1371/journal.ppat.1001178
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