Cargando…

Optimization of ex vivo hematopoietic stem cell expansion in intermittent dynamic cultures

For the ex vivo expansion of CD34(+) cells, culture conditions were optimized using cytokine cocktails and media change methods. In addition, static, orbital-shake, and stirred cultures were compared. After cultivation, total cell expansion, immunophenotypes, clonogenic ability, and metabolite conce...

Descripción completa

Detalles Bibliográficos
Autores principales: Choi, Yong-Soo, Noh, Sang-Eun, Lim, Sang-Min, Kim, Dong-il
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974206/
https://www.ncbi.nlm.nih.gov/pubmed/20652621
http://dx.doi.org/10.1007/s10529-010-0355-0