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Optimization of ex vivo hematopoietic stem cell expansion in intermittent dynamic cultures
For the ex vivo expansion of CD34(+) cells, culture conditions were optimized using cytokine cocktails and media change methods. In addition, static, orbital-shake, and stirred cultures were compared. After cultivation, total cell expansion, immunophenotypes, clonogenic ability, and metabolite conce...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974206/ https://www.ncbi.nlm.nih.gov/pubmed/20652621 http://dx.doi.org/10.1007/s10529-010-0355-0 |