Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

[Image: see text] Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Exten...

Descripción completa

Detalles Bibliográficos
Autores principales: McGregor, Lynn M., Gorin, David J., Dumelin, Christoph E., Liu, David R.
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society 2010
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974369/
https://www.ncbi.nlm.nih.gov/pubmed/20949943
http://dx.doi.org/10.1021/ja107677q
_version_ 1782190877260644352
author McGregor, Lynn M.
Gorin, David J.
Dumelin, Christoph E.
Liu, David R.
author_facet McGregor, Lynn M.
Gorin, David J.
Dumelin, Christoph E.
Liu, David R.
author_sort McGregor, Lynn M.
collection PubMed
description [Image: see text] Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences.
format Text
id pubmed-2974369
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-29743692010-11-05 Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment McGregor, Lynn M. Gorin, David J. Dumelin, Christoph E. Liu, David R. J Am Chem Soc [Image: see text] Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein−ligand pairs out of 67 599 possible sequences. American Chemical Society 2010-10-15 2010-11-10 /pmc/articles/PMC2974369/ /pubmed/20949943 http://dx.doi.org/10.1021/ja107677q Text en Copyright © 2010 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle McGregor, Lynn M.
Gorin, David J.
Dumelin, Christoph E.
Liu, David R.
Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
title Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
title_full Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
title_fullStr Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
title_full_unstemmed Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
title_short Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment
title_sort interaction-dependent pcr: identification of ligand−target pairs from libraries of ligands and libraries of targets in a single solution-phase experiment
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2974369/
https://www.ncbi.nlm.nih.gov/pubmed/20949943
http://dx.doi.org/10.1021/ja107677q
work_keys_str_mv AT mcgregorlynnm interactiondependentpcridentificationofligandtargetpairsfromlibrariesofligandsandlibrariesoftargetsinasinglesolutionphaseexperiment
AT gorindavidj interactiondependentpcridentificationofligandtargetpairsfromlibrariesofligandsandlibrariesoftargetsinasinglesolutionphaseexperiment
AT dumelinchristophe interactiondependentpcridentificationofligandtargetpairsfromlibrariesofligandsandlibrariesoftargetsinasinglesolutionphaseexperiment
AT liudavidr interactiondependentpcridentificationofligandtargetpairsfromlibrariesofligandsandlibrariesoftargetsinasinglesolutionphaseexperiment

Ejemplares similares