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An Engineered Methanogenic Pathway Derived from the Domains Bacteria and Archaea

A plasmid-based expression system wherein mekB was fused to a constitutive Methanosarcina acetivorans promoter was used to express MekB, a broad-specificity esterase from Pseudomonas veronii, in M. acetivorans. The engineered strain had 80-fold greater esterase activity than wild-type M. acetivorans...

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Detalles Bibliográficos
Autores principales: Lessner, Daniel J., Lhu, Lexan, Wahal, Christopher S., Ferry, James G.
Formato: Texto
Lenguaje:English
Publicado: American Society of Microbiology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2975365/
https://www.ncbi.nlm.nih.gov/pubmed/21060738
http://dx.doi.org/10.1128/mBio.00243-10