Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation

BACKGROUND: Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins have been traditionally expr...

Descripción completa

Detalles Bibliográficos
Autores principales: Fong, Baley A, Wood, David W
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978133/
https://www.ncbi.nlm.nih.gov/pubmed/20959011
http://dx.doi.org/10.1186/1475-2859-9-77

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978133/
https://www.ncbi.nlm.nih.gov/pubmed/20959011
http://dx.doi.org/10.1186/1475-2859-9-77

Ejemplares similares