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Expression and purification of ELP-intein-tagged target proteins in high cell density E. coli fermentation
BACKGROUND: Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins have been traditionally expr...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978133/ https://www.ncbi.nlm.nih.gov/pubmed/20959011 http://dx.doi.org/10.1186/1475-2859-9-77 |