Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers

The integrated cell culture quantitative reverse transcriptase PCR (ICC/qRT-PCR) method is used in our lab to detect enteroviruses in environmental waters. Typically we utilize monolayers of 3 cell lines; buffalo green monkey kidney (BGM), human colonic carcinoma (CACO-2) and African rhesus monkey k...

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Detalles Bibliográficos
Autores principales: Balkin, Helene B, Margolin, Aaron B
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978159/
https://www.ncbi.nlm.nih.gov/pubmed/20974002
http://dx.doi.org/10.1186/1743-422X-7-282

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978159/
https://www.ncbi.nlm.nih.gov/pubmed/20974002
http://dx.doi.org/10.1186/1743-422X-7-282

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