DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme

To cut DNA at their target sites, restriction enzymes assemble into different oligomeric structures. The Ecl18kI endonuclease in the crystal is arranged as a tetramer made of two dimers each bound to a DNA copy. However, free in solution Ecl18kI is a dimer. To find out whether the Ecl18kI dimer or t...

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Detalles Bibliográficos
Autores principales: Zaremba, Mindaugas, Owsicka, Amelia, Tamulaitis, Gintautas, Sasnauskas, Giedrius, Shlyakhtenko, Luda S., Lushnikov, Alexander Y., Lyubchenko, Yuri L., Laurens, Niels, van den Broek, Bram, Wuite, Gijs J. L., Siksnys, Virginijus
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978343/
https://www.ncbi.nlm.nih.gov/pubmed/20571089
http://dx.doi.org/10.1093/nar/gkq560

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978343/
https://www.ncbi.nlm.nih.gov/pubmed/20571089
http://dx.doi.org/10.1093/nar/gkq560

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