Subtle gene modification in mouse ES cells: evidence for incorporation of unmodified oligonucleotides without induction of DNA damage

Gene targeting by single-stranded oligodeoxyribonucleotides (ssODNs) is a promising tool for site-specific gene modification in mouse embryonic stem cells (ESCs). We have developed an ESC line carrying a mutant EGFP reporter gene to monitor gene correction events shortly after exposure to ssODNs. We...

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Detalles Bibliográficos
Autores principales: Aarts, Marieke, te Riele, Hein
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Genome Integrity, Repair and Replication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978364/
https://www.ncbi.nlm.nih.gov/pubmed/20601408
http://dx.doi.org/10.1093/nar/gkq589

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2978364/
https://www.ncbi.nlm.nih.gov/pubmed/20601408
http://dx.doi.org/10.1093/nar/gkq589

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