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Tracing antigen signatures in the human IgE repertoire

Allergen recognition by IgE antibodies is a key event in allergic inflammation. In this study, the IgE IGHV repertoires of individuals with allergy to the major birch pollen allergen, Bet v 1, were analyzed over a four years period of allergen exposure by RT-PCR and sequencing of cDNA. Approximately...

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Autores principales: Marth, Katharina, Novatchkova, Maria, Focke-Tejkl, Margarete, Jenisch, Stefan, Jäger, Siegfried, Kabelitz, Dieter, Valenta, Rudolf
Formato: Texto
Lenguaje:English
Publicado: Pergamon Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2981859/
https://www.ncbi.nlm.nih.gov/pubmed/20573403
http://dx.doi.org/10.1016/j.molimm.2010.05.285
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author Marth, Katharina
Novatchkova, Maria
Focke-Tejkl, Margarete
Jenisch, Stefan
Jäger, Siegfried
Kabelitz, Dieter
Valenta, Rudolf
author_facet Marth, Katharina
Novatchkova, Maria
Focke-Tejkl, Margarete
Jenisch, Stefan
Jäger, Siegfried
Kabelitz, Dieter
Valenta, Rudolf
author_sort Marth, Katharina
collection PubMed
description Allergen recognition by IgE antibodies is a key event in allergic inflammation. In this study, the IgE IGHV repertoires of individuals with allergy to the major birch pollen allergen, Bet v 1, were analyzed over a four years period of allergen exposure by RT-PCR and sequencing of cDNA. Approximately half of the IgE transcripts represented non-redundant sequences, which belonged to seventeen different IGHV genes. Most variable regions contained somatic mutations but also non-mutated sequences were identified. There was no evidence for relevant increases of somatic mutations over time of allergen exposure. Highly similar IgE variable regions were found after four years of allergen exposure in the same and in genetically non-related individuals. Our results indicate that allergens select and shape a limited number of similar IgE variable regions in the human IgE repertoire.
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spelling pubmed-29818592010-12-07 Tracing antigen signatures in the human IgE repertoire Marth, Katharina Novatchkova, Maria Focke-Tejkl, Margarete Jenisch, Stefan Jäger, Siegfried Kabelitz, Dieter Valenta, Rudolf Mol Immunol Article Allergen recognition by IgE antibodies is a key event in allergic inflammation. In this study, the IgE IGHV repertoires of individuals with allergy to the major birch pollen allergen, Bet v 1, were analyzed over a four years period of allergen exposure by RT-PCR and sequencing of cDNA. Approximately half of the IgE transcripts represented non-redundant sequences, which belonged to seventeen different IGHV genes. Most variable regions contained somatic mutations but also non-mutated sequences were identified. There was no evidence for relevant increases of somatic mutations over time of allergen exposure. Highly similar IgE variable regions were found after four years of allergen exposure in the same and in genetically non-related individuals. Our results indicate that allergens select and shape a limited number of similar IgE variable regions in the human IgE repertoire. Pergamon Press 2010-08 /pmc/articles/PMC2981859/ /pubmed/20573403 http://dx.doi.org/10.1016/j.molimm.2010.05.285 Text en © 2010 Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Article
Marth, Katharina
Novatchkova, Maria
Focke-Tejkl, Margarete
Jenisch, Stefan
Jäger, Siegfried
Kabelitz, Dieter
Valenta, Rudolf
Tracing antigen signatures in the human IgE repertoire
title Tracing antigen signatures in the human IgE repertoire
title_full Tracing antigen signatures in the human IgE repertoire
title_fullStr Tracing antigen signatures in the human IgE repertoire
title_full_unstemmed Tracing antigen signatures in the human IgE repertoire
title_short Tracing antigen signatures in the human IgE repertoire
title_sort tracing antigen signatures in the human ige repertoire
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2981859/
https://www.ncbi.nlm.nih.gov/pubmed/20573403
http://dx.doi.org/10.1016/j.molimm.2010.05.285
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