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Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity
BACKGROUND: The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2984594/ https://www.ncbi.nlm.nih.gov/pubmed/21029474 http://dx.doi.org/10.1186/1743-422X-7-294 |
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author | Sun, Aijun Li, Yanpeng Wang, Jingyan Su, Shuai Chen, Hongjun Zhu, Hongfei Ding, Jiabo Cui, Zhizhong |
author_facet | Sun, Aijun Li, Yanpeng Wang, Jingyan Su, Shuai Chen, Hongjun Zhu, Hongfei Ding, Jiabo Cui, Zhizhong |
author_sort | Sun, Aijun |
collection | PubMed |
description | BACKGROUND: The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA. RESULTS: Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101Δ(A+C) was constructed. The present experiments indicated that GX0101Δ(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bi-directional promoter between 1.8-kb mRNA and pp38 genes of MDV. CONCLUSION: These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV. |
format | Text |
id | pubmed-2984594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29845942010-11-19 Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity Sun, Aijun Li, Yanpeng Wang, Jingyan Su, Shuai Chen, Hongjun Zhu, Hongfei Ding, Jiabo Cui, Zhizhong Virol J Research BACKGROUND: The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA. RESULTS: Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101Δ(A+C) was constructed. The present experiments indicated that GX0101Δ(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bi-directional promoter between 1.8-kb mRNA and pp38 genes of MDV. CONCLUSION: These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV. BioMed Central 2010-10-29 /pmc/articles/PMC2984594/ /pubmed/21029474 http://dx.doi.org/10.1186/1743-422X-7-294 Text en Copyright ©2010 Sun et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Sun, Aijun Li, Yanpeng Wang, Jingyan Su, Shuai Chen, Hongjun Zhu, Hongfei Ding, Jiabo Cui, Zhizhong Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity |
title | Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity |
title_full | Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity |
title_fullStr | Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity |
title_full_unstemmed | Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity |
title_short | Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity |
title_sort | deletion of 1.8-kb mrna of marek's disease virus decreases its replication ability but not oncogenicity |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2984594/ https://www.ncbi.nlm.nih.gov/pubmed/21029474 http://dx.doi.org/10.1186/1743-422X-7-294 |
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