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Selective (13)C labeling of nucleotides for large RNA NMR spectroscopy using an E. coli strain disabled in the TCA cycle

Escherichia coli (E. coli) is an ideal organism to tailor-make labeled nucleotides for biophysical studies of RNA. Recently, we showed that adding labeled formate enhanced the isotopic enrichment at protonated carbon sites in nucleotides. In this paper, we show that growth of a mutant E. coli strain...

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Detalles Bibliográficos
Autores principales: Thakur, Chandar S., Sama, Jacob N., Jackson, Melantha E., Chen, Bin, Dayie, T. Kwaku
Formato: Texto
Lenguaje:English
Publicado: Springer Netherlands 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2988204/
https://www.ncbi.nlm.nih.gov/pubmed/21057854
http://dx.doi.org/10.1007/s10858-010-9454-4