An Improved Protocol for N-Glycosylation Analysis of Gel-Separated Sialylated Glycoproteins by MALDI-TOF/TOF

Different glycoforms of some proteins have been identified as differential spots for certain diseases in 2-DE, indicating disease-related glycosylation changes. It is routine to determine the site-specific glycosylation of nonsialylated N-glycoproteins from a single gel spot, but some obstacles stil...

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Detalles Bibliográficos
Autores principales: Hao, Piliang, Ren, Yan, Xie, Yongming
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994013/
https://www.ncbi.nlm.nih.gov/pubmed/21124746
http://dx.doi.org/10.1371/journal.pone.0015096

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994013/
https://www.ncbi.nlm.nih.gov/pubmed/21124746
http://dx.doi.org/10.1371/journal.pone.0015096

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