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A serine-type protease activity of human lens βA3-crystallin is responsible for its autodegradation
PURPOSE: The purpose of the study was to determine whether the autodegradation of human βA3-crystallin is due to its intrinsic protease activity. METHODS: Recombinant His-tagged human βA3-crystallin was expressed in E. coli and purified by a Ni(+2)-affinity column chromatographic method. To determin...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Molecular Vision
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994418/ https://www.ncbi.nlm.nih.gov/pubmed/21139689 |