By-passing in vitro screening—next generation sequencing technologies applied to antibody display and in silico candidate selection

In recent years, unprecedented DNA sequencing capacity provided by next generation sequencing (NGS) has revolutionized genomic research. Combining the Illumina sequencing platform and a scFv library designed to confine diversity to both CDR3, >1.9 × 10(7) sequences have been generated. This appro...

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Detalles Bibliográficos
Autores principales: Ravn, U., Gueneau, F., Baerlocher, L., Osteras, M., Desmurs, M., Malinge, P., Magistrelli, G., Farinelli, L., Kosco-Vilbois, M.H., Fischer, N.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995085/
https://www.ncbi.nlm.nih.gov/pubmed/20846958
http://dx.doi.org/10.1093/nar/gkq789

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995085/
https://www.ncbi.nlm.nih.gov/pubmed/20846958
http://dx.doi.org/10.1093/nar/gkq789

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