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A one-step method for quantitative determination of uracil in DNA by real-time PCR

Uracil may occur in DNA due to either cytosine deamination or thymine replacing incorporation. Its quantitative characterization is important in assessing DNA damages in cells with perturbed thymidylate metabolism or within different DNA segments involved in immunoglobulin gene diversification. The...

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Detalles Bibliográficos
Autores principales: Horváth, András, Vértessy, Beáta G.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995087/
https://www.ncbi.nlm.nih.gov/pubmed/20864450
http://dx.doi.org/10.1093/nar/gkq815