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Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice

Theiler’s murine encephalomyelitis virus-induced demyelination (TMEVD) and experimental allergic encephalomyelitis (EAE) are the principal animal models of multiple sclerosis (MS). Previously we provided evidence that Tmevd2 and Eae3 may represent either a shared susceptibility locus or members of a...

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Autores principales: Spach, Karen M., Case, Laure K., Noubade, Rajkumar, Petersen, Chase B., McElvany, Ben, Zalik, Nathan, Hickey, William F., Blankenhorn, Elizabeth P., Teuscher, Cory
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995842/
https://www.ncbi.nlm.nih.gov/pubmed/20861860
http://dx.doi.org/10.1038/gene.2010.40
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author Spach, Karen M.
Case, Laure K.
Noubade, Rajkumar
Petersen, Chase B.
McElvany, Ben
Zalik, Nathan
Hickey, William F.
Blankenhorn, Elizabeth P.
Teuscher, Cory
author_facet Spach, Karen M.
Case, Laure K.
Noubade, Rajkumar
Petersen, Chase B.
McElvany, Ben
Zalik, Nathan
Hickey, William F.
Blankenhorn, Elizabeth P.
Teuscher, Cory
author_sort Spach, Karen M.
collection PubMed
description Theiler’s murine encephalomyelitis virus-induced demyelination (TMEVD) and experimental allergic encephalomyelitis (EAE) are the principal animal models of multiple sclerosis (MS). Previously we provided evidence that Tmevd2 and Eae3 may represent either a shared susceptibility locus or members of a gene complex controlling susceptibility to CNS inflammatory demyelinating disease. To explore the genetic relationship between Tmevd2 and Eae3, we generated a D2.C-Tmevd2 interval-specific congenic (ISC) line and three overlapping interval-specific recombinant congenic (ISRC) lines in which the Tmevd2 resistant allele from BALB/cByJ was introgressed onto the TMEVD-susceptible DBA/2J background. These mice, all H2(d), were studied for susceptibility to EAE elicited by immunization with PLP(180–199). Compared to DBA/2J mice, D2.C-Tmevd2 mice developed a significantly less severe clinical disease course and EAE pathology in the spinal cord, confirming the existence of Eae3 and its linkage to Tmevd2 in this strain combination. Compare to DBA/2J and D2.C-Tmevd2, all three ISRC lines exhibited clinical disease courses of intermediate severity. Neither differences in ex vivo antigen-specific cytokine nor proliferative responses uniquely cosegregated with differences in disease severity. These results indicate that multiple QTL within the Tmevd2/Eae3 interval influence EAE severity, one of which includes a homology region for a QTL found in MS by admixture mapping.
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spelling pubmed-29958422011-06-01 Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice Spach, Karen M. Case, Laure K. Noubade, Rajkumar Petersen, Chase B. McElvany, Ben Zalik, Nathan Hickey, William F. Blankenhorn, Elizabeth P. Teuscher, Cory Genes Immun Article Theiler’s murine encephalomyelitis virus-induced demyelination (TMEVD) and experimental allergic encephalomyelitis (EAE) are the principal animal models of multiple sclerosis (MS). Previously we provided evidence that Tmevd2 and Eae3 may represent either a shared susceptibility locus or members of a gene complex controlling susceptibility to CNS inflammatory demyelinating disease. To explore the genetic relationship between Tmevd2 and Eae3, we generated a D2.C-Tmevd2 interval-specific congenic (ISC) line and three overlapping interval-specific recombinant congenic (ISRC) lines in which the Tmevd2 resistant allele from BALB/cByJ was introgressed onto the TMEVD-susceptible DBA/2J background. These mice, all H2(d), were studied for susceptibility to EAE elicited by immunization with PLP(180–199). Compared to DBA/2J mice, D2.C-Tmevd2 mice developed a significantly less severe clinical disease course and EAE pathology in the spinal cord, confirming the existence of Eae3 and its linkage to Tmevd2 in this strain combination. Compare to DBA/2J and D2.C-Tmevd2, all three ISRC lines exhibited clinical disease courses of intermediate severity. Neither differences in ex vivo antigen-specific cytokine nor proliferative responses uniquely cosegregated with differences in disease severity. These results indicate that multiple QTL within the Tmevd2/Eae3 interval influence EAE severity, one of which includes a homology region for a QTL found in MS by admixture mapping. 2010-09-23 2010-12 /pmc/articles/PMC2995842/ /pubmed/20861860 http://dx.doi.org/10.1038/gene.2010.40 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Spach, Karen M.
Case, Laure K.
Noubade, Rajkumar
Petersen, Chase B.
McElvany, Ben
Zalik, Nathan
Hickey, William F.
Blankenhorn, Elizabeth P.
Teuscher, Cory
Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
title Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
title_full Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
title_fullStr Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
title_full_unstemmed Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
title_short Multiple Linked Quantitative Trait Loci within the Tmevd2/Eae3 Interval Control the Severity of Experimental Allergic Encephalomyelitis in DBA/2J Mice
title_sort multiple linked quantitative trait loci within the tmevd2/eae3 interval control the severity of experimental allergic encephalomyelitis in dba/2j mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2995842/
https://www.ncbi.nlm.nih.gov/pubmed/20861860
http://dx.doi.org/10.1038/gene.2010.40
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