Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples

BACKGROUND: The template switching PCR (TS-PCR) method of cDNA synthesis represents one of the most straightforward approaches to generating full length cDNA for sequencing efforts. However, when applied to very small RNA samples, such as those obtained from tens or hundreds of cells, this approach...

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Detalles Bibliográficos
Autores principales: Kapteyn, Jeremy, He, Ruifeng, McDowell, Eric T, Gang, David R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996941/
https://www.ncbi.nlm.nih.gov/pubmed/20598146
http://dx.doi.org/10.1186/1471-2164-11-413

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996941/
https://www.ncbi.nlm.nih.gov/pubmed/20598146
http://dx.doi.org/10.1186/1471-2164-11-413

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