Systematic analysis of off-target effects in an RNAi screen reveals microRNAs affecting sensitivity to TRAIL-induced apoptosis

BACKGROUND: RNA inhibition by siRNAs is a frequently used approach to identify genes required for specific biological processes. However RNAi screening using siRNAs is hampered by non-specific or off target effects of the siRNAs, making it difficult to separate genuine hits from false positives. It...

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Detalles Bibliográficos
Autores principales: Sudbery, Ian, Enright, Anton J, Fraser, Andrew G, Dunham, Ian
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996961/
https://www.ncbi.nlm.nih.gov/pubmed/20230625
http://dx.doi.org/10.1186/1471-2164-11-175

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2996961/
https://www.ncbi.nlm.nih.gov/pubmed/20230625
http://dx.doi.org/10.1186/1471-2164-11-175

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