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Expression in E. coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase

BACKGROUND: Chitin synthase 3a (CHS3a) from Botrytis cinerea (Bc) catalyses the multiple transfer of N-acetylglucosamine (GlcNAc) residues to the growing chitin chain. Chitin, a β-1,4 linked GlcNAc homopolymer, is an essential cell wall component of filamentous fungi. Chitin synthase, processive mem...

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Detalles Bibliográficos
Autores principales: Magellan, Hervé, Drujon, Thierry, Thellend, Annie, Piffeteau, Annie, Becker, Hubert F
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2997770/
https://www.ncbi.nlm.nih.gov/pubmed/21070667
http://dx.doi.org/10.1186/1756-0500-3-299