Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification

In vitro reconstitution of the bacteriophage T4 replication machinery provides a novel system for fast and processive isothermal DNA amplification. We have characterized this system in two formats: (i) in circular nicking endonuclease-dependent amplification (cNDA), the T4 replisome is supplemented...

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Detalles Bibliográficos
Autores principales: Schaerli, Yolanda, Stein, Viktor, Spiering, Michelle M., Benkovic, Stephen J., Abell, Chris, Hollfelder, Florian
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001092/
https://www.ncbi.nlm.nih.gov/pubmed/20921065
http://dx.doi.org/10.1093/nar/gkq795

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001092/
https://www.ncbi.nlm.nih.gov/pubmed/20921065
http://dx.doi.org/10.1093/nar/gkq795

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