Cargando…

Isothermal DNA amplification using the T4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification

In vitro reconstitution of the bacteriophage T4 replication machinery provides a novel system for fast and processive isothermal DNA amplification. We have characterized this system in two formats: (i) in circular nicking endonuclease-dependent amplification (cNDA), the T4 replisome is supplemented...

Descripción completa

Detalles Bibliográficos
Autores principales:	Schaerli, Yolanda, Stein, Viktor, Spiering, Michelle M., Benkovic, Stephen J., Abell, Chris, Hollfelder, Florian
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001092/ https://www.ncbi.nlm.nih.gov/pubmed/20921065 http://dx.doi.org/10.1093/nar/gkq795

Ejemplares similares

Primase-based whole genome amplification
por: Li, Ying, et al.
Publicado: (2008)

Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)
por: Kiesling, Traci, et al.
Publicado: (2007)

Simultaneous amplification and screening of whole plasmids using the T7 bacteriophage replisome
por: Xu, Yan, et al.
Publicado: (2006)

Optimization of turn-back primers in isothermal amplification
por: Kimura, Yasumasa, et al.
Publicado: (2011)

Sequence dependence of isothermal DNA amplification via EXPAR
por: Qian, Jifeng, et al.
Publicado: (2012)

Types and Applications of Nicking Enzyme-Combined Isothermal Amplification
por: Cao, Siyu, et al.
Publicado: (2022)

Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification
por: Yi, Jizu, et al.
Publicado: (2006)

Genome amplification of single sperm using multiple displacement amplification
por: Jiang, Zhengwen, et al.
Publicado: (2005)

Labeling of unique sequences in double-stranded DNA at sites of vicinal nicks generated by nicking endonucleases
por: Kuhn, Heiko, et al.
Publicado: (2008)

Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification
por: Murakami, Taku, et al.
Publicado: (2009)

Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
por: Dahl, Fredrik, et al.
Publicado: (2005)

Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification
por: Watanabe, Takaaki, et al.
Publicado: (2011)

Boosting riboswitch efficiency by RNA amplification
por: Emadpour, Masoumeh, et al.
Publicado: (2015)

Impact of whole genome amplification on analysis of copy number variants
por: Pugh, T. J., et al.
Publicado: (2008)

Low temperature isothermal amplification of microsatellites drastically reduces stutter artifact formation and improves microsatellite instability detection in cancer
por: Daunay, Antoine, et al.
Publicado: (2019)

Asymmetric exponential amplification reaction on a toehold/biotin featured template: an ultrasensitive and specific strategy for isothermal microRNAs analysis
por: Chen, Jun, et al.
Publicado: (2016)

Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging
por: Miura, Fumihito, et al.
Publicado: (2012)

The use of multiple displacement amplification to amplify complex DNA libraries
por: Fullwood, Melissa J., et al.
Publicado: (2008)

Enzymatic signal amplification of molecular beacons for sensitive DNA detection
por: Li, Jianwei Jeffery, et al.
Publicado: (2008)

PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
por: Suslov, Oleg, et al.
Publicado: (2005)

Tapping diversity lost in transformations—in vitro amplification of ligation reactions
por: Christ, Daniel, et al.
Publicado: (2006)

Amplification ratio control system for copy number variation genotyping
por: Guthrie, Philip A. I., et al.
Publicado: (2011)

Enhanced sequencing coverage with digital droplet multiple displacement amplification
por: Sidore, Angus M., et al.
Publicado: (2016)

Rolling circle extension-assisted loop-mediated isothermal amplification (Rol-LAMP) method for locus-specific and visible detection of RNA N(6)-methyladenosine
por: Li, Jiexin, et al.
Publicado: (2023)

NAIMA: target amplification strategy allowing quantitative on-chip detection of GMOs
por: Morisset, Dany, et al.
Publicado: (2008)

PieceMaker: selection of DNA fragments for selector-guided multiplex amplification
por: Stenberg, Johan, et al.
Publicado: (2005)

Tri-nucleotide threading for parallel amplification of minute amounts of genomic DNA
por: Pettersson, Erik, et al.
Publicado: (2006)

Effective transcriptome amplification for expression profiling on sense-oriented oligonucleotide microarrays
por: Schlingemann, Joerg, et al.
Publicado: (2005)

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
por: Ruijter, J. M., et al.
Publicado: (2009)

Identification of small gains and losses in single cells after whole genome amplification on tiling oligo arrays
por: Geigl, Jochen B., et al.
Publicado: (2009)

Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA
por: Gutjahr, Alice, et al.
Publicado: (2014)

A Novel Ultrasensitive ECL Sensor for DNA Detection Based on Nicking Endonuclease-Assisted Target Recycling Amplification, Rolling Circle Amplification and Hemin/G-Quadruplex
por: Luo, Fukang, et al.
Publicado: (2015)

Lack of correlation between reaction speed and analytical sensitivity in isothermal amplification reveals the value of digital methods for optimization: validation using digital real-time RT-LAMP
por: Khorosheva, Eugenia M., et al.
Publicado: (2016)

Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input
por: Chen, Danping, et al.
Publicado: (2019)

Headloop suppression PCR and its application to selective amplification of methylated DNA sequences
por: Rand, Keith N., et al.
Publicado: (2005)

An unnatural base pair system for efficient PCR amplification and functionalization of DNA molecules
por: Kimoto, Michiko, et al.
Publicado: (2009)

Rolling circle amplification-mediated hairpin RNA (RMHR) library construction in plants
por: Wang, Lei, et al.
Publicado: (2008)

A nuclease-polymerase chain reaction enables amplification of probes used for capture-based DNA target enrichment
por: Leong, Ka Wai, et al.
Publicado: (2019)

Restriction Endonuclease-Based Assays for DNA Detection and Isothermal Exponential Signal Amplification
por: Smith, Maria, et al.
Publicado: (2020)

Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification
por: Zhang, Kaixiang, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_c09gqlgtqhfn8kh7ntml70sfa2