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Impact of Reference Gene Selection for Target Gene Normalization on Experimental Outcome Using Real-Time qRT-PCR in Adipocytes

BACKGROUND: With the current rise in obesity-related morbidities, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become a widely used method for assessment of genes expressed and regulated by adipocytes. In order to measure accurate changes in relative gene expr...

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Detalles Bibliográficos
Autores principales: Ferguson, Bradley S., Nam, Heesun, Hopkins, Robin G., Morrison, Ron F.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001470/
https://www.ncbi.nlm.nih.gov/pubmed/21179435
http://dx.doi.org/10.1371/journal.pone.0015208