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Impact of Reference Gene Selection for Target Gene Normalization on Experimental Outcome Using Real-Time qRT-PCR in Adipocytes
BACKGROUND: With the current rise in obesity-related morbidities, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) has become a widely used method for assessment of genes expressed and regulated by adipocytes. In order to measure accurate changes in relative gene expr...
Autores principales: | Ferguson, Bradley S., Nam, Heesun, Hopkins, Robin G., Morrison, Ron F. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3001470/ https://www.ncbi.nlm.nih.gov/pubmed/21179435 http://dx.doi.org/10.1371/journal.pone.0015208 |
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