Cargando…

Construction and Characterization of Insect Cell-Derived Influenza VLP: Cell Binding, Fusion, and EGFP Incorporation

We have constructed virus-like particles (VLPs) harboring hemagglutinin (HA), neuraminidase (NA), matrix protein 1 (M1) ,and proton channel protein (M2) using baculovirus as a vector in the SF9 insect cell. The size of the expressed VLP was estimated to be ~100 nm by light scattering experiment and...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pan, Yi-Shin, Wei, Hung-Ju, Chang, Chung-Chieh, Lin, Chung-Hung, Wei, Ting-Shyang, Wu, Suh-Chin, Chang, Ding-Kwo
Formato:	Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004410/ https://www.ncbi.nlm.nih.gov/pubmed/21197092 http://dx.doi.org/10.1155/2010/506363

Ejemplares similares

Dissecting insect cell heterogeneity during influenza VLP production using single-cell transcriptomics
por: Silvano, Marco, et al.
Publicado: (2023)

Gene Expression Analysis of Adapted Insect Cells during Influenza VLP Production Using RNA-Sequencing
por: Silvano, Marco, et al.
Publicado: (2022)

Human Papillomavirus Type16- L1 VLP Production in Insect Cells
por: Abdoli, Asghar, et al.
Publicado: (2013)

Virus-like particle (VLP)-based vaccines for pandemic influenza: Performance of a VLP vaccine during the 2009 influenza pandemic
por: López-Macías, Constantino
Publicado: (2012)

Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production
por: Lynch, Alisson G, et al.
Publicado: (2010)

Process development for pandemic influenza VLP vaccine production using a baculovirus expression system
por: Lai, Chia-Chun, et al.
Publicado: (2019)

Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells
por: Blecha, Andreas, et al.
Publicado: (2005)

Generation of an Lpar1-EGFP Fusion Knock-in Transgenic Mouse Line
por: Rivera, Richard, et al.
Publicado: (2021)

Construction of the plasmid for expression of EGFP-M-IL-2(88Arg, 125Ala) fusion protein and the anti-tumor effects exerted by the fusion protein in HeLa-60 cells
por: Shao, Guangcan, et al.
Publicado: (2021)

Nanobody‑horseradish peroxidase and -EGFP fusions as reagents to detect porcine parvovirus in the immunoassays
por: Lu, Qizhong, et al.
Publicado: (2020)

Critical assessment of influenza VLP production in Sf9 and HEK293 expression systems
por: Thompson, Christine M, et al.
Publicado: (2015)

Evaluation of eGFP expression in the ChAT-eGFP transgenic mouse brain
por: Gamage, Rashmi, et al.
Publicado: (2023)

Construction of an infectious clone of Zika virus stably expressing an EGFP marker in a eukaryotic expression system
por: Gao, Jing, et al.
Publicado: (2021)

Construction of recombinant pEGFP-N1-hPer2 plasmid and its expression in osteosarcoma cells
por: CHENG, ANYUAN, et al.
Publicado: (2016)

Construction of a recombinant pIRES2-EGFP-ARTS plasmid and its effect on LX-2 cells
por: Xu, Feifan, et al.
Publicado: (2017)

Interaction between Borrelia miyamotoi variable major proteins Vlp15/16 and Vlp18 with plasminogen and complement
por: Schmidt, Frederik L., et al.
Publicado: (2021)

Membrane interaction and structure of the transmembrane domain of influenza hemagglutinin and its fusion peptide complex
por: Chang, Ding-Kwo, et al.
Publicado: (2008)

Comparison of Immune Responses to Different Versions of VLP Associated Stabilized RSV Pre-Fusion F Protein
por: Cullen, Lori M., et al.
Publicado: (2019)

Comparisons of Antibody Populations in Different Pre-Fusion F VLP-Immunized Cotton Rat Dams and Their Offspring
por: Cullen, Lori M., et al.
Publicado: (2020)

On the Effect of Thermodynamic Equilibrium on the Assembly Efficiency of Complex Multi-Layered Virus-Like Particles (VLP): the Case of Rotavirus VLP
por: Roldão, António, et al.
Publicado: (2012)

VLP‐factory™ and ADDomer(©): Self‐assembling Virus‐Like Particle (VLP) Technologies for Multiple Protein and Peptide Epitope Display
por: Sari‐Ak, Duygu, et al.
Publicado: (2021)

Generation of Col2a1-EGFP iPS Cells for Monitoring Chondrogenic Differentiation
por: Saito, Taku, et al.
Publicado: (2013)

A Luciferase-EGFP Reporter System for the Evaluation of DNA Methylation in Mammalian Cells
por: Wang, X. X., et al.
Publicado: (2021)

Enhancing performance of ZnO dye-sensitized solar cells by incorporation of multiwalled carbon nanotubes
por: Chang, Wei-Chen, et al.
Publicado: (2012)

Mapping eGFP Oligomer Mobility in Living Cell Nuclei
por: Dross, Nicolas, et al.
Publicado: (2009)

RNA Phage VLP-Based Vaccine Platforms
por: Peabody, David S., et al.
Publicado: (2021)

Erythro-VLP: Erythrocyte Virus-Like-Particles
por: Himbert, Sebastian, et al.
Publicado: (2021)

Construction of eGFP-Tagged Senecavirus A for Facilitating Virus Neutralization Test and Antiviral Assay
por: Liu, Fuxiao, et al.
Publicado: (2020)

Improved fusion protein expression of EGFP via the mutation of both Kozak and the initial ATG codon
por: Dai, Chao, et al.
Publicado: (2007)

The Use of Distinctive Monoclonal Antibodies in FMD VLP- and P1-Based Blocking ELISA for the Seromonitoring of Vaccinated Swine
por: Lee, Heng-Wei, et al.
Publicado: (2022)

Constructing a Mouse Oct4 Promoter/EGFP Vector, as a Whole-Cellular Reporter to Monitor the Pluripotent State of Cells
por: Ghorbani, Reza, et al.
Publicado: (2013)

Construction and characterization of EGFP reporter plasmid harboring putative human RAX promoter for in vitro monitoring of retinal progenitor cells identity
por: Atefi, Atefeh, et al.
Publicado: (2021)

The Dark Side of EGFP: Defective Polyubiquitination
por: Baens, Mathijs, et al.
Publicado: (2006)

rsEGFP2 enables fast RESOLFT nanoscopy of living cells
por: Grotjohann, Tim, et al.
Publicado: (2012)

Fluorescent proteins such as eGFP lead to catalytic oxidative stress in cells()
por: Ganini, Douglas, et al.
Publicado: (2017)

Construction of Recombinant Bacmid Containing M2e-Ctxb and Producing the Fusion Protein in Insect Cell Lines
por: Mirzaei, Nima, et al.
Publicado: (2014)

Zika virus-like particle (VLP) based vaccine
por: Boigard, Hélène, et al.
Publicado: (2017)

Large-scale production and purification of VLP-based vaccines
por: Vicente, Tiago, et al.
Publicado: (2011)

Virus-like Particle (VLP) Vaccines for Cancer Immunotherapy
por: Ruzzi, Francesca, et al.
Publicado: (2023)

Potential utility of eGFP-expressing NOG mice (NOG-EGFP) as a high purity cancer sampling system
por: Shima, Kentaro, et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_pntvsiinhjmu2j19md6vj6tids