Cargando…

High yield expression of leptospirosis vaccine candidates LigA and LipL32 in the methylotrophic yeast Pichia pastoris

BACKGROUND: Leptospirosis, a zoonosis caused by Leptospira spp., is recognized as an emergent infectious disease. Due to the lack of adequate diagnostic tools, vaccines are an attractive intervention strategy. Recombinant proteins produced in Escherichia coli have demonstrated promising results, alb...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hartwig, Daiane D, Oliveira, Thaís L, Seixas, Fabiana K, Forster, Karine M, Rizzi, Caroline, Hartleben, Cláudia P, McBride, Alan JA, Dellagostin, Odir A
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2010
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004844/ https://www.ncbi.nlm.nih.gov/pubmed/21134266 http://dx.doi.org/10.1186/1475-2859-9-98

Ejemplares similares

LipL41 and LigA/LigB Gene Silencing on a LipL32 Knockout Leptospira interrogans Reveals the Impact of Multiple Mutations on Virulence
por: Fernandes, Luis Guilherme V., et al.
Publicado: (2023)

Evaluation of LipL32 and LigA/LigB Knockdown Mutants in Leptospira interrogans Serovar Copenhageni: Impacts to Proteome and Virulence
por: Fernandes, Luis G. V., et al.
Publicado: (2022)

The use of halloysite clay and carboxyl-functionalised multi-walled carbon nanotubes for recombinant LipL32 antigen delivery enhanced the IgG response
por: Hartwig, Daiane D, et al.
Publicado: (2015)

Immunoprotective properties of recombinant LigA and LigB in a hamster model of acute leptospirosis
por: Evangelista, Karen V., et al.
Publicado: (2017)

Immune response in hamsters immunised with a recombinant fragment of LigA from Leptospira interrogans, associated with carrier molecules
por: Oliveira, Thaís L, et al.
Publicado: (2016)

Evaluation of truncated LipL32 expressed by Escherichia coli and Pichia pastoris for serodiagnosis of Leptospira infection in rodents
por: SHIOKAWA, Kanae, et al.
Publicado: (2015)

Peptide specific monoclonal antibodies of Leptospiral LigA for acute diagnosis of leptospirosis
por: Kanagavel, Murugesan, et al.
Publicado: (2017)

LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis
por: Conrad, Neida L., et al.
Publicado: (2017)

Sequence analysis of LipL41 and LipL21: Prospective Outer Membrane Proteins (OMPs) in early diagnosing leptospirosis
por: Mosquill, Muzaffar, et al.
Publicado: (2022)

Recombinant production of bacterial toxins and their derivatives in the methylotrophic yeast Pichia pastoris
por: Gurkan, Cemal, et al.
Publicado: (2005)

Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris
por: Ivashov, Vasyl A., et al.
Publicado: (2013)

Identification of triacylglycerol and steryl ester synthases of the methylotrophic yeast Pichia pastoris
por: Ivashov, Vasyl A., et al.
Publicado: (2013)

Evaluation of Leptospira interrogans knockdown mutants for LipL32, LipL41, LipL21, and OmpL1 proteins
por: Fernandes, Luis G. V., et al.
Publicado: (2023)

A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris
por: Weninger, Astrid, et al.
Publicado: (2015)

RETRACTED ARTICLE: Expression and preliminary characterization of the potential vaccine candidate LipL32 of leptospirosis
por: Govindan, Pothiaraj, et al.
Publicado: (2021)

Xanthan Gum as an Adjuvant in a Subunit Vaccine Preparation against Leptospirosis
por: Bacelo, Katia L., et al.
Publicado: (2014)

Genome-scale metabolic reconstruction and in silico analysis of methylotrophic yeast Pichia pastoris for strain improvement
por: Chung, Bevan KS, et al.
Publicado: (2010)

GC-Rich DNA Elements Enable Replication Origin Activity in the Methylotrophic Yeast Pichia pastoris
por: Liachko, Ivan, et al.
Publicado: (2014)

Orthologous promoters from related methylotrophic yeasts surpass expression of endogenous promoters of Pichia pastoris
por: Vogl, Thomas, et al.
Publicado: (2020)

Cloning of a synthetic chimeric gene containing recombinant Mycoplasma hyopneumoniae antigens for expression in Pichia pastoris
por: Gomes, Charles, et al.
Publicado: (2014)

Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
por: Joseph, Siju, et al.
Publicado: (2012)

Cloning and characterization of PAS5: a gene required for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris
Publicado: (1993)

Expression and Purification of Functionally Active Recombinant Human Alpha 1-Antitrypsin in Methylotrophic Yeast Pichia pastoris
por: Arjmand, Sareh, et al.
Publicado: (2011)

Recombinant human N-acetylgalactosamine-6-sulfate sulfatase (GALNS) produced in the methylotrophic yeast Pichia pastoris
por: Rodríguez-López, Alexander, et al.
Publicado: (2016)

Analysis of N‐glycosylation in fungal l‐amino acid oxidases expressed in the methylotrophic yeast Pichia pastoris
por: Heß, Marc Christian, et al.
Publicado: (2021)

DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
por: Forster, Karine M, et al.
Publicado: (2015)

Development and evaluation of latex agglutination test coating with recombinant antigen, LipL32 for serodiagnosis of human leptospirosis
por: Thongsukkaeng, Kotchakorn, et al.
Publicado: (2018)

Immunohistochemical detection of Lp25 and LipL32 proteins in skeletal and cardiac muscles of fatal human leptospirosis
por: Iglezias, Silvia D’Andretta, et al.
Publicado: (2020)

Identification of Novel Inhibitors of Escherichia coli DNA Ligase (LigA)
por: Alomari, Arqam, et al.
Publicado: (2021)

The leptospiral LipL21 and LipL41 proteins exhibit a broad spectrum of interactions with host cell components
por: MB, Takahashi, et al.
Publicado: (2021)

Cytokine expression profile in hamsters immunized with OmpL37 from Leptospirainterrogans in different vaccine formulations
por: Oliveira, Thaís, et al.
Publicado: (2014)

The polyglutamine protein ataxin-3 enables normal growth under heat shock conditions in the methylotrophic yeast Pichia pastoris
por: Bonanomi, Marcella, et al.
Publicado: (2017)

Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool
por: Forster, Karine M., et al.
Publicado: (2013)

The Terminal Immunoglobulin-Like Repeats of LigA and LigB of Leptospira Enhance Their Binding to Gelatin Binding Domain of Fibronectin and Host Cells
por: Lin, Yi-Pin, et al.
Publicado: (2010)

Immunoinformatic Study of Recombinant LigA/BCon1-5 Antigen and Evaluation of Its Diagnostic Potential in Primary and Secondary Binding Tests for Serodiagnosis of Porcine Leptospirosis
por: Behera, Sujit Kumar, et al.
Publicado: (2021)

Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin
por: Kranthi, Balla Venkata, et al.
Publicado: (2006)

Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
por: Cos, Oriol, et al.
Publicado: (2006)

Leptospira noguchii and Human and Animal Leptospirosis, Southern Brazil
por: Silva, Éverton F., et al.
Publicado: (2009)

Evolutionary Implication of Outer Membrane Lipoprotein-Encoding Genes ompL1, lipL32 and lipL41 of Pathogenic Leptospira Species
por: Vedhagiri, K., et al.
Publicado: (2009)

COMPARISON OF 16S rRNA-PCR-RFLP, LipL32-PCR AND OmpL1-PCR METHODS IN THE DIAGNOSIS OF LEPTOSPIROSIS
por: GÖKMEN, Tülin GÜVEN, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_kj12d17l53ti77jcbt652iv6hs